In the use of pipettes, the basic tool of molecular biotechnology, the concepts of disinfection and sterilization are often mixed. The difference between the two is that disinfection only requires that the live bacteria on the pipette be controlled within a certain range to achieve a harmless level, while sterilization requires the elimination of all live bacteria. Sterilization requires more processing than disinfection. Below, Pipette Tips Manufacturers introduces the sterilization strategy of pipettes:
1. Chemical disinfection. Simply put, wipe the outer surface of the pipette with alcohol, etc., and then dry it. This should be possible for all pipette brands, otherwise, the material of the housing is too bad!
2. Ultraviolet disinfection. The surface of the pipette is irradiated with UV light, which sterilizes by destroying the DNA structure of cells. The duration of UV disinfection depends on the intensity of radiation and the resistance of bacteria to UV rays. Most brands of pipettes can be sterilized with UV light, but need to check with the supplier in advance.
3. Sterilize the pipette at high temperature and autoclave. Generally speaking, the working conditions of high temperature and high-pressure sterilization of pipettes are 102.9kPa (1.05kg/cm2), 121℃, 20 minutes. The high-temperature and high-pressure sterilization of pipettes are divided into whole sterilization and half sterilization. It is absolutely possible that most brands of pipettes can be sterilized in half, but whether the whole pipe can be sterilized, the user must contact the supplier for business confirmation. On the market, products such as manual pipettes can be sterilized as a whole. However, there are a few points to remind here: First, except for individual applications, such as infectious virus research, most customers do not need to sterilize whole tubes; second, for products of the same brand, whole tube sterilization is often more expensive than half tube sterilization Yes, customers need to make decisions based on their needs.