Pipette Tips Manufacturers Introduce Tips For Using Pipettes

The appearance rate of pipettes in laboratories, especially in chemical and pharmaceutical laboratories, is very high. Some researchers even have to hold the pipette hundreds of times a day to pipette liquids. Improving the speed and precision with which researchers use pipettes can greatly improve laboratory productivity. Below, Pipette Tips Manufacturers describe tips for using pipettes:

1. The most important thing is to choose the appropriate pipette size and tips. According to the amount of liquid to be pipetted and the characteristics of the liquid, select the appropriate size of pipette and tip. If a pipette with a large volume is used to absorb a small amount of liquid, it will easily cause a suction deviation, especially when using a manual pipette, the error will be greater. The key to the tip is to match the pipette to ensure tightness to reduce sample loss due to tip leakage.

2. Select the appropriate pipette according to the density of the liquid. More viscous liquids require slower pipetting and work in reverse pipette mode; volatile liquids require faster pipetting and work in reverse mode. When pipetting liquids with a large difference in density from water, the pipette needs to be calibrated first.

3. Before the actual pipetting, aspirate the liquid several times to pre-wet the tip. This reduces pipetting errors due to evaporation or liquid retention.

4. During operation, the tip should not be immersed in the liquid too deeply, and the residence time should not be too short or too long, about 1 second is more appropriate.

5. Pay attention to the tilt angle of the pipette during operation, and try to keep the pipette as vertical as possible.

6. Try to ensure that the pipette, tips, and liquid are at room temperature. If special temperature conditions are required due to the characteristics of the sample, try to make the temperature of the pipette and the liquid as close as possible to avoid errors caused by excessive temperature differences.

7. Use consistent and stable speed and pressure when handling pipettes and samples. When aspirating manually, pause for one to two seconds after each aspiration to allow the liquid level to stabilize in the tip. Develop good operating habits, and clean the instrument in time after each use of the pipette, so as to avoid the dirt growing in the instrument from affecting the normal operation of the instrument. The instrument is calibrated regularly to ensure its accuracy.